Title : Wortmannin inactivates phosphoinositide 3-kinase by covalent modification of Lys-802, a residue involved in the phosphate transfer reaction.

Pub. Date : 1996 Apr

PMID : 8657148






5 Functional Relationships(s)
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1 We report here the identification of the site of interaction of wortmannin on the catalytic subunit of PI 3-kinase, p110alpha. Wortmannin phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha Homo sapiens
2 At physiological pH (6.5 to 8) wortmannin reacted specifically with p110alpha. Wortmannin phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha Homo sapiens
3 Proteolytic fragments of wortmannin-treated, recombinant p110alpha were mapped with anti-wortmannin and anti-p110alpha peptide antibodies, thus limiting the target site within a 10-kDa fragment, colocalizing with the ATP-binding site. Wortmannin phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha Homo sapiens
4 Proteolytic fragments of wortmannin-treated, recombinant p110alpha were mapped with anti-wortmannin and anti-p110alpha peptide antibodies, thus limiting the target site within a 10-kDa fragment, colocalizing with the ATP-binding site. Wortmannin phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha Homo sapiens
5 Proteolytic fragments of wortmannin-treated, recombinant p110alpha were mapped with anti-wortmannin and anti-p110alpha peptide antibodies, thus limiting the target site within a 10-kDa fragment, colocalizing with the ATP-binding site. Wortmannin phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha Homo sapiens