Title : [Isolation and purification of pregnancy-associated plasma protein A].

Pub. Date : 1996 Jan

PMID : 8576617






2 Functional Relationships(s)
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Compound Name
Protein Name
Organism
1 Secondary PAPP-A containing fractions were applied to a Heparin-Sepharose column and eluted by a stepwise increase in NaCl 0.15, 0.30 and 0.60 M in 0.05 M Tris-HCl buffer, pH 7.8. Heparin pappalysin 1 Homo sapiens
2 Thirdly the pooled fractions which contained PAPP-A after Heparin-Sepharose affinity chromatography were applied to DEAE-Sephacel Chromatography and eluted by a stepwise increase in NaCl 0.15, 0.30 and 0.45 M in 0.01 M acetate buffer, pH 5.5. Heparin pappalysin 1 Homo sapiens