Pub. Date : 1993 Apr
PMID : 8473524
3 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | A retroviral vector (BAG) was used to transfer human prostaglandin H synthase (PGHS-1) gene into a human endothelial cell line for enhancement of PGI2 synthesis. | Epoprostenol | prostaglandin-endoperoxide synthase 1 | Homo sapiens |
| 2 | Cells infected with BAG containing PGHS-1 cDNA in the sense orientation relative to the retroviral promoter (PGHS(S)) expressed a 30-fold increase in mRNA but, due to a reading frame shift, did not show an increase in PGHS protein or in PGI2 synthesis, while those with PGHS-1 in reverse orientation relative to the viral promoter (PGHS(R)), produced a > 10-fold increase in PGHS mRNA over the control (169 +/- 22 vs 14.8 +/- 1.2 amol/micrograms RNA) with a concordant increase in PGHS protein (5.82 +/- 1.07 vs 0.23 +/- 0.04 ng/mg protein) and enzyme activity. | Epoprostenol | prostaglandin-endoperoxide synthase 1 | Homo sapiens |
| 3 | These findings indicate that transfer of PGHS-1 gene into vascular cells enhances PGI2 synthesis and may be a useful strategy for restoring thromboprotective property of damaged blood vessels. | Epoprostenol | prostaglandin-endoperoxide synthase 1 | Homo sapiens |