Title : Spectrofluorimetric method for the quantification of 7-hydroxycoumarin in urine and plasma using both extracted and unextracted samples.

Pub. Date : 1993 Feb

PMID : 8442515






1 Functional Relationships(s)
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1 The amount of 7-OHC conjugated to glucuronide and excreted in the urine, was determined following treatment with beta-glucuronidase (5000 U ml(-1);1 U = 16.67 nkat) for 30 min at 37 degrees C. This allows the urinary concentration of free, total and conjugated 7-OHC to be determined. 7-HYDROXYCHOLESTEROL glucuronidase beta Homo sapiens