Title : GTP-dependent association of Raf-1 with Ha-Ras: identification of Raf as a target downstream of Ras in mammalian cells.

Pub. Date : 1993 Sep 15

PMID : 8378348






3 Functional Relationships(s)
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Protein Name
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1 GTP-dependent association of Raf-1 with Ha-Ras: identification of Raf as a target downstream of Ras in mammalian cells. Guanosine Triphosphate Harvey rat sarcoma virus oncogene Mus musculus
2 In an effort to identify the Ras-associated downstream proteins, we added recombinant Ha-Ras in a GTP-bound form to cell-free lysates and used several antibodies against Ras to immunoprecipitate Ras complexes. Guanosine Triphosphate Harvey rat sarcoma virus oncogene Mus musculus
3 The GTP-dependent association of Ha-Ras with Raf-1 was observed with lysates of various types of cultured cells, including NIH 3T3, pheochromocytoma (PC) 12, Ba/F3, and Jurkat T cells, and also with crude extracts from rat brain. Guanosine Triphosphate Harvey rat sarcoma virus oncogene Mus musculus