Title : Guanidinium chloride induction of partial unfolding in amide proton exchange in RNase A.

Pub. Date : 1993 Nov 5

PMID : 8235609






3 Functional Relationships(s)
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Compound Name
Protein Name
Organism
1 Guanidinium chloride induction of partial unfolding in amide proton exchange in RNase A. Amides ribonuclease A family member 1, pancreatic Homo sapiens
2 Amide (NH) proton exchange rates were measured in 0.0 to 0.7 M guanidinium chloride (GdmCl) for 23 slowly exchanging peptide NH protons of ribonuclease A (RNase A) at pH* 5.5 (uncorrected pH measured in D2O), 34 degrees C. The purpose was to find out whether GdmCl induces exchange through binding to exchange intermediates that are partly or wholly unfolded. Amides ribonuclease A family member 1, pancreatic Homo sapiens
3 Amide (NH) proton exchange rates were measured in 0.0 to 0.7 M guanidinium chloride (GdmCl) for 23 slowly exchanging peptide NH protons of ribonuclease A (RNase A) at pH* 5.5 (uncorrected pH measured in D2O), 34 degrees C. The purpose was to find out whether GdmCl induces exchange through binding to exchange intermediates that are partly or wholly unfolded. Amides ribonuclease A family member 1, pancreatic Homo sapiens