Title : Cdc25p, the guanine nucleotide exchange factor for the Ras proteins of Saccharomyces cerevisiae, promotes exchange by stabilizing Ras in a nucleotide-free state.

Pub. Date : 1994 Jun 17

PMID : 8206969






2 Functional Relationships(s)
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1 Using Ras2p and a catalytic fragment of Cdc25p, both expressed in and purified from Escherichia coli, we determined that Cdc25p has a Km for Ras2p-GDP of 160 nM and a maximal rate of 0.20 s-1. Guanosine Diphosphate Ras family guanine nucleotide exchange factor CDC25 Saccharomyces cerevisiae S288C
2 The Km of Cdc25p for Ras2p complexed to GTP is 3-fold greater than that for Ras2p complexed to GDP. Guanosine Diphosphate Ras family guanine nucleotide exchange factor CDC25 Saccharomyces cerevisiae S288C