Title : Effect of 5-fluoro- and 5-bromouracil substitution on the translation of human thymidylate synthase mRNA.

Pub. Date : 1994 Jun 10

PMID : 8206932






6 Functional Relationships(s)
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1 The synthesis of thymidylate synthase (TS) from 5-fluorouracil (FUra)- and 5-bromouracil (BrUra)-substituted mRNAs was examined to investigate the effect of incorporation of uracil (Ura) analogs on translation. Uracil thymidylate synthetase Homo sapiens
2 The synthesis of thymidylate synthase (TS) from 5-fluorouracil (FUra)- and 5-bromouracil (BrUra)-substituted mRNAs was examined to investigate the effect of incorporation of uracil (Ura) analogs on translation. Uracil thymidylate synthetase Homo sapiens
3 The synthesis of thymidylate synthase (TS) from 5-fluorouracil (FUra)- and 5-bromouracil (BrUra)-substituted mRNAs was examined to investigate the effect of incorporation of uracil (Ura) analogs on translation. Uracil thymidylate synthetase Homo sapiens
4 The synthesis of thymidylate synthase (TS) from 5-fluorouracil (FUra)- and 5-bromouracil (BrUra)-substituted mRNAs was examined to investigate the effect of incorporation of uracil (Ura) analogs on translation. Uracil thymidylate synthetase Homo sapiens
5 Human TS cDNA was transcribed in the presence of Ura-, FUra-, or BrUTP to obtain 100% substituted mRNA. Uracil thymidylate synthetase Homo sapiens
6 Time courses of TS formation revealed a characteristic peak which occurred at 45 min for the Ura- and FUra-RNAs and at 2 h for the BrUra-RNA. Uracil thymidylate synthetase Homo sapiens