Title : Oxidation of monohydric phenol substrates by tyrosinase: effect of dithiothreitol on kinetics.

Pub. Date : 1994 Nov 15

PMID : 7998927






1 Functional Relationships(s)
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1 We have demonstrated three actions of DTT on tyrosinase-catalysed reactions: (1) direct reduction of the copper at the active site of the enzyme; (2) generation of secondary, oxidizable species by adduct formation with the o-quinone reaction product, 4-MOB, which leads to an increase in the total oxygen utilization by the reaction system; and (3) reversible inhibition of the enzyme. Oxygen tyrosinase Homo sapiens