Title : Regulation of GAD expression in islets of Langerhans occurs both at the mRNA and protein level.

Pub. Date : 1994 Jun

PMID : 7926271






4 Functional Relationships(s)
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1 Using immunoprecipitations and analyses of enzyme activity, an increase in glutamate decarboxylase was detected in rat islets cultured at a glucose concentration of 11 mmol/l compared with those cultured at 5.6 mmol/l glucose. Glucose glutamate-ammonia ligase Rattus norvegicus
2 Using immunoprecipitations and analyses of enzyme activity, an increase in glutamate decarboxylase was detected in rat islets cultured at a glucose concentration of 11 mmol/l compared with those cultured at 5.6 mmol/l glucose. Glucose glutamate-ammonia ligase Rattus norvegicus
3 Comparative quantitation in a phosphor imager revealed a significantly higher (82%, P < 0.005) content of glutamate decarboxylase mRNA in islets cultured at 11 mmol/l glucose. Glucose glutamate-ammonia ligase Rattus norvegicus
4 Islets cultured at 11 mmol/l glucose in the presence of 40 U/ml of interleukin-1 beta, showed a 63% decrease (P < 0.005) in enzyme activity compared with those cultured at 11 mmol/l glucose alone, and similar decreases were noted on analysis of glutamate decarboxylase biosynthesis and mRNA. Glucose glutamate-ammonia ligase Rattus norvegicus