Title : Interaction studies between elongation factor Tu and anthraniloyl-fluorescent analogues of guanyl nucleotides.

Pub. Date : 1995 Jan 15

PMID : 7851417






3 Functional Relationships(s)
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1 A fluorescent analogue of GDP, the 3"-O-anthraniloyl-GDP (anl-GDP) was demonstrated to bind to the elongation factor Tu (EF-Tu) with an affinity even higher than that of the parent nucleotide. Guanosine Diphosphate eukaryotic translation elongation factor 1 alpha 1 Homo sapiens
2 A fluorescent analogue of GDP, the 3"-O-anthraniloyl-GDP (anl-GDP) was demonstrated to bind to the elongation factor Tu (EF-Tu) with an affinity even higher than that of the parent nucleotide. Guanosine Diphosphate eukaryotic translation elongation factor 1 alpha 1 Homo sapiens
3 In this way, it was also easily proven that, in the presence of aurodox (N-methylkirromycin), an antibiotic impairing EF-Tu biological function, the exchange kinetics between the protein-bound labeled GDP and the natural nucleotide was faster. Guanosine Diphosphate eukaryotic translation elongation factor 1 alpha 1 Homo sapiens