Title : Bound fibrinogen distribution on stimulated platelets. Examination by confocal scanning laser microscopy.

Pub. Date : 1995 Sep

PMID : 7677179






8 Functional Relationships(s)
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1 When fluorescein isothiocyanate (FITC)-streptavidin was added to ADP-stimulated platelets 1 minute after biotinylated fibrinogen binding at 22 degrees C, bound fibrinogen was found in variously sized patches on the cell surface. Fluorescein-5-isothiocyanate fibrinogen beta chain Homo sapiens
2 When fluorescein isothiocyanate (FITC)-streptavidin was added to ADP-stimulated platelets 1 minute after biotinylated fibrinogen binding at 22 degrees C, bound fibrinogen was found in variously sized patches on the cell surface. Fluorescein-5-isothiocyanate fibrinogen beta chain Homo sapiens
3 When fluorescein isothiocyanate (FITC)-streptavidin was added to ADP-stimulated platelets 1 minute after biotinylated fibrinogen binding at 22 degrees C, bound fibrinogen was found in variously sized patches on the cell surface. Fluorescein-5-isothiocyanate fibrinogen beta chain Homo sapiens
4 Additional studies using either biotinylated fibrinogen that had been prelabeled with FITC-streptavidin or FITC-labeled fibrinogen revealed similar patterns of platelet-associated fibrinogen clearance and redistribution. Fluorescein-5-isothiocyanate fibrinogen beta chain Homo sapiens
5 Additional studies using either biotinylated fibrinogen that had been prelabeled with FITC-streptavidin or FITC-labeled fibrinogen revealed similar patterns of platelet-associated fibrinogen clearance and redistribution. Fluorescein-5-isothiocyanate fibrinogen beta chain Homo sapiens
6 Additional studies using either biotinylated fibrinogen that had been prelabeled with FITC-streptavidin or FITC-labeled fibrinogen revealed similar patterns of platelet-associated fibrinogen clearance and redistribution. Fluorescein-5-isothiocyanate fibrinogen beta chain Homo sapiens
7 Since surface-cleared fibrinogen was accessible to exogenous FITC-streptavidin under conditions that did not lead to platelet permeabilization, the data suggest fibrinogen deposition in compartments that are accessible to the extracellular milieu. Fluorescein-5-isothiocyanate fibrinogen beta chain Homo sapiens
8 Since surface-cleared fibrinogen was accessible to exogenous FITC-streptavidin under conditions that did not lead to platelet permeabilization, the data suggest fibrinogen deposition in compartments that are accessible to the extracellular milieu. Fluorescein-5-isothiocyanate fibrinogen beta chain Homo sapiens