Title : Analysis of ethylene signal-transduction kinetics associated with seedling-growth response and chitinase induction in wild-type and mutant arabidopsis.

Pub. Date : 1995 Jun

PMID : 7610160






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1 Analysis of ethylene signal-transduction kinetics associated with seedling-growth response and chitinase induction in wild-type and mutant arabidopsis. ethylene chitinase Arabidopsis thaliana
2 Kinetic aspects of ethylene-mediated signal transduction leading to seedling-growth inhibition and chitinase induction in Arabidopsis were investigated by the introduction of defined mutations in components of these pathways. ethylene chitinase Arabidopsis thaliana
3 Dose-response analysis of wild-type responses indicated that the rate-limiting steps for seedling responses and Arabidopsis basic-chitinase induction displayed Michaelis-Menten kinetics with apparent dissociation constants of the response (Kr) of 0.1 and 1.4 microL L-1 ethylene, respectively. ethylene chitinase Arabidopsis thaliana
4 In the ethylene-insensitive etr1-1 and ein2-32 mutant lines, both Arabidopsis basic-chitinase induction and seedling-growth responses were completely disrupted, whereas the weaker etr1-2 allele eliminated the chitinase-induction response but only partially disrupted the seedling responses. ethylene chitinase Arabidopsis thaliana
5 In the ethylene-insensitive etr1-1 and ein2-32 mutant lines, both Arabidopsis basic-chitinase induction and seedling-growth responses were completely disrupted, whereas the weaker etr1-2 allele eliminated the chitinase-induction response but only partially disrupted the seedling responses. ethylene chitinase Arabidopsis thaliana
6 A heterologous reporter gene containing the chitinase promoter from bean (bean basic-chitinase-beta-glucuronidase) displayed subsensitive kinetics (Kr 120 microL L-1 ethylene) compared to the response of the endogenous basic-chitinase response (Kr 1.4 microL L-1 ethylene). ethylene chitinase Arabidopsis thaliana
7 A heterologous reporter gene containing the chitinase promoter from bean (bean basic-chitinase-beta-glucuronidase) displayed subsensitive kinetics (Kr 120 microL L-1 ethylene) compared to the response of the endogenous basic-chitinase response (Kr 1.4 microL L-1 ethylene). ethylene chitinase Arabidopsis thaliana
8 A heterologous reporter gene containing the chitinase promoter from bean (bean basic-chitinase-beta-glucuronidase) displayed subsensitive kinetics (Kr 120 microL L-1 ethylene) compared to the response of the endogenous basic-chitinase response (Kr 1.4 microL L-1 ethylene). ethylene chitinase Arabidopsis thaliana
9 A heterologous reporter gene containing the chitinase promoter from bean (bean basic-chitinase-beta-glucuronidase) displayed subsensitive kinetics (Kr 120 microL L-1 ethylene) compared to the response of the endogenous basic-chitinase response (Kr 1.4 microL L-1 ethylene). ethylene chitinase Arabidopsis thaliana