Title : Polypeptide chain initiation in eukaryotes: reversibility of the ternary complex-forming reaction.

Pub. Date : 1983 Mar

PMID : 6572381






9 Functional Relationships(s)
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1 However, at physiological Mg2+ concentrations, GDP is known to have approximately equal to 100-fold greater affinity than GTP for eIF-2 and eIF-2 is believed to be released in the form of an eIF-2.GDP complex. Guanosine Triphosphate eukaryotic translation initiation factor 2 subunit beta Homo sapiens
2 However, at physiological Mg2+ concentrations, GDP is known to have approximately equal to 100-fold greater affinity than GTP for eIF-2 and eIF-2 is believed to be released in the form of an eIF-2.GDP complex. Guanosine Triphosphate eukaryotic translation initiation factor 2 subunit beta Homo sapiens
3 However, at physiological Mg2+ concentrations, GDP is known to have approximately equal to 100-fold greater affinity than GTP for eIF-2 and eIF-2 is believed to be released in the form of an eIF-2.GDP complex. Guanosine Triphosphate eukaryotic translation initiation factor 2 subunit beta Homo sapiens
4 Previously, we have shown that initiation factor SP (for eIF-2-stimulating protein) promotes the exchange of eIF-2-bound GDP for GTP and catalyzes ternary complex formation in the presence of Met-tRNAi. Guanosine Triphosphate eukaryotic translation initiation factor 2 subunit beta Homo sapiens
5 Previously, we have shown that initiation factor SP (for eIF-2-stimulating protein) promotes the exchange of eIF-2-bound GDP for GTP and catalyzes ternary complex formation in the presence of Met-tRNAi. Guanosine Triphosphate eukaryotic translation initiation factor 2 subunit beta Homo sapiens
6 We further show that, in the presence of Mg2+ and catalytic amounts of SP, ternary complex formation conforms to the overall reversible reaction eIF-2.GDP + GTP + Met-tRNAi in equilibrium eIF-2.GTP.Met-tRNAi + GDP. Guanosine Triphosphate eukaryotic translation initiation factor 2 subunit beta Homo sapiens
7 We further show that, in the presence of Mg2+ and catalytic amounts of SP, ternary complex formation conforms to the overall reversible reaction eIF-2.GDP + GTP + Met-tRNAi in equilibrium eIF-2.GTP.Met-tRNAi + GDP. Guanosine Triphosphate eukaryotic translation initiation factor 2 subunit beta Homo sapiens
8 We further show that, in the presence of Mg2+ and catalytic amounts of SP, ternary complex formation conforms to the overall reversible reaction eIF-2.GDP + GTP + Met-tRNAi in equilibrium eIF-2.GTP.Met-tRNAi + GDP. Guanosine Triphosphate eukaryotic translation initiation factor 2 subunit beta Homo sapiens
9 We further show that, in the presence of Mg2+ and catalytic amounts of SP, ternary complex formation conforms to the overall reversible reaction eIF-2.GDP + GTP + Met-tRNAi in equilibrium eIF-2.GTP.Met-tRNAi + GDP. Guanosine Triphosphate eukaryotic translation initiation factor 2 subunit beta Homo sapiens