Title : Protease mitogenic response of chick embryo fibroblasts and receptor binding/processing of human alpha-thrombin.

Pub. Date : 1981 Mar 25

PMID : 6259143






1 Functional Relationships(s)
Download
Sentence
Compound Name
Protein Name
Organism
1 Thrombin associated with the former was released into the media when the cells were incubated at 0-4 degrees C with hirudin or hydroxylamine, or transferred to the insensitive compartment when incubated at 22 degrees C. Finally, confluent cultures of fibroblasts bind 2-3 x 10(4) 125I-alpha-thrombin molecules/cell with an apparent binding constant, i.e. Kd, of 0.7 nM (a true Kd could not be determined because of the irreversible nature of thrombin binding). Hydroxylamine coagulation factor II, thrombin Homo sapiens