Title : Assay for glutamine synthetase activity.

Pub. Date : 1973 Dec

PMID : 4149766






1 Functional Relationships(s)
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1 The use of phosphoenolpyruvate plus pyruvate kinase as an ATP-generating system in the assay for glutamine synthetase activity via the formation of gamma-glutamylhydroxamate from glutamate and hydroxylamine with crude tissue preparations is shown to give values far in excess of the true glutamine synthetase activity of the tissue. Hydroxylamine glutamate-ammonia ligase Homo sapiens