Title : Identification of ribosome-bound eukaryotic initiation factor 2.GDP binary complex as an intermediate in polypeptide chain initiation reaction.

Pub. Date : 1986 Jun 15

PMID : 3711104






3 Functional Relationships(s)
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1 Studies on the formation and release of the eukaryotic initiation factor (eIF)-2.GDP binary complex formed during eIF-5-mediated assembly of an 80 S initiation complex have been carried out. Guanosine Diphosphate eukaryotic translation initiation factor 5 Homo sapiens
2 Incubation of a 40 S initiation complex with eIF-5, in the presence or absence of 60 S ribosomal subunits at 25 degrees C, causes rapid and quantitative hydrolysis of ribosome-bound GTP to form an eIF-2.GDP binary complex and Pi. Guanosine Diphosphate eukaryotic translation initiation factor 5 Homo sapiens
3 The eIF-2.GDP binary complex can however be released from ribosome by subjecting the eIF-5-catalyzed reaction products to either longer periods of incubation at 37 degrees C or sucrose gradient centrifugation. Guanosine Diphosphate eukaryotic translation initiation factor 5 Homo sapiens