Title : Enzyme-triggered click chemistry combined with surface-enhanced Raman spectroscopy for the simple and sensitive detection of alkaline phosphatase activity from complex biological samples.

Pub. Date : 2022 May 30

PMID : 35536049






3 Functional Relationships(s)
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1 Then, AA could reduce Cu(II) to produce Cu(I), which plays the role of a catalyst to promote the click reaction of azide terephthalic acid (ATA) and 4-acetylene biphenyl (4-AB), resulting in the SERS signal intensity of free 4-AB in the solution was clearly reduced along with the click reaction carried on, and showed a quantitative relationship with the concentration of ALP. sers alkaline phosphatase, placental Homo sapiens
2 As a proof of concept, the new developed SERS-click strategy was applied to the specific determination of the ALP activity in clinical serum samples, cellular lysate samples and the ALP inhibitor assessment successfully, indicating that the proposed ALP-triggered click chemistry assay has a significant potential application in medical diagnosis. sers alkaline phosphatase, placental Homo sapiens
3 As a proof of concept, the new developed SERS-click strategy was applied to the specific determination of the ALP activity in clinical serum samples, cellular lysate samples and the ALP inhibitor assessment successfully, indicating that the proposed ALP-triggered click chemistry assay has a significant potential application in medical diagnosis. sers alkaline phosphatase, placental Homo sapiens