Title : Effect of Ultraviolet B Irradiation on Melanin Content Accompanied by the Activation of p62/GATA4-Mediated Premature Senescence in HaCaT Cells.

Pub. Date : 2022 Jan-Mar

PMID : 35185418






2 Functional Relationships(s)
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1 NaOH method was used for melanin content assay, cellular tyrosinase (TYR) activity was determined by 3,4-Dihydroxy-L-phenylalanine (L-DOPA) oxidation to dopachrome, premature senescence was analyzed by senescence-associated beta-galactosidase (SA-beta-gal) staining kit, and the levels of p21, p16, p62, and GATA4 proteins were detected by Western blotting. Levodopa tyrosinase Homo sapiens
2 NaOH method was used for melanin content assay, cellular tyrosinase (TYR) activity was determined by 3,4-Dihydroxy-L-phenylalanine (L-DOPA) oxidation to dopachrome, premature senescence was analyzed by senescence-associated beta-galactosidase (SA-beta-gal) staining kit, and the levels of p21, p16, p62, and GATA4 proteins were detected by Western blotting. Levodopa tyrosinase Homo sapiens