Title : Determination of sorbitol dehydrogenase in microsamples of human serum.

Pub. Date : 2021 Dec 1

PMID : 34517598






4 Functional Relationships(s)
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1 The determination relies on the oxidation of NADH cofactor, which is used by SDH reacting with its substrates. NAD sorbitol dehydrogenase Homo sapiens
2 The concentration of cofactors (NADH, NAD+) and substrates (fructose, sorbitol) for SDH determination at a strip was optimized via internally-calibrated amperometric assays at a chitosan/nitrogen-doped carbon nanotube electrode. NAD sorbitol dehydrogenase Homo sapiens
3 The concentration of cofactors (NADH, NAD+) and substrates (fructose, sorbitol) for SDH determination at a strip was optimized via internally-calibrated amperometric assays at a chitosan/nitrogen-doped carbon nanotube electrode. NAD sorbitol dehydrogenase Homo sapiens
4 The assays yielded kinetic parameters Km and kcat and demonstrated higher apparent affinity of SDH for NADH and fructose than NAD+ and sorbitol. NAD sorbitol dehydrogenase Homo sapiens