Title : ALISA: A microplate assay to measure protein thiol redox state.

Pub. Date : 2021 Oct

PMID : 34418513






3 Functional Relationships(s)
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1 After validating the assay principle (i.e., increased target specific reversible thiol oxidation increases the ratio), we used ALISA to determine whether fertilisation-a fundamental biological process-changes Akt, a serine/threonine protein kinase, specific reversible thiol oxidation. Sulfhydryl Compounds v-akt murine thymoma viral oncogene homolog 1 S homeolog Xenopus laevis
2 After validating the assay principle (i.e., increased target specific reversible thiol oxidation increases the ratio), we used ALISA to determine whether fertilisation-a fundamental biological process-changes Akt, a serine/threonine protein kinase, specific reversible thiol oxidation. Sulfhydryl Compounds v-akt murine thymoma viral oncogene homolog 1 S homeolog Xenopus laevis
3 Fertilisation significantly decreases Akt specific reversible thiol oxidation in Xenopus laevis 2-cell zygotes compared to unfertilised eggs. Sulfhydryl Compounds v-akt murine thymoma viral oncogene homolog 1 S homeolog Xenopus laevis