Title : Improving the Sensitivity of the miRNA Assay Coupled with the Mismatched Catalytic Hairpin Assembly Reaction by Optimization of Hairpin Annealing Conditions.

Pub. Date : 2021 May 4

PMID : 33899474






1 Functional Relationships(s)
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1 In this work, it was shown that the introduction of salts such as NaCl and MgCl2 to HP1/HP2 annealing solutions sharply reduces the background in mCHA and simultaneously increases the signal-to-background (S/B) ratio. Sodium Chloride chromobox 5 Homo sapiens