Pub. Date : 2020
PMID : 33679289
4 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | A Novel SOD1 Intermediate Oligomer, Role of Free Thiols and Disulfide Exchange. | Disulfides | superoxide dismutase 1 | Homo sapiens |
| 2 | Wild-type human SOD1 forms a highly conserved intra-molecular disulfide bond between C57-C146, and in its native state is greatly stabilized by binding one copper and one zinc atom per monomer rendering the protein dimeric. | Disulfides | superoxide dismutase 1 | Homo sapiens |
| 3 | Here we further investigate the role of reduction of the native C57-C146 disulfide bond in fibrillation of wild-type hSOD1, firstly through removal of free thiols by paired mutations C6A, C111S (AS-SOD1), and secondly in seeded fibrillation reactions modulated by reductant tris (2-carboxyethyl) phosphine (TCEP). | Disulfides | superoxide dismutase 1 | Homo sapiens |
| 4 | Fibrillation of AS-SOD1 was dependent upon disulfide reduction and showed classic lag and exponential growth phases compared with wild-type hSOD1 whose fibrillation trajectories were typically somewhat perturbed. | Disulfides | superoxide dismutase 1 | Homo sapiens |