Title : Phenobarbital induction of cytochrome P-450 b,e genes is dependent on protein synthesis.

Pub. Date : 1988 Mar-Apr

PMID : 3356413






8 Functional Relationships(s)
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1 Phenobarbital induction of cytochrome P-450 b,e genes is dependent on protein synthesis. Phenobarbital cytochrome P450 family 4 subfamily F member 3 Homo sapiens
2 Phenobarbital induces liver cytochrome P-450 b,e proteins mainly by increasing the rate of transcription of these genes. Phenobarbital cytochrome P450 family 4 subfamily F member 3 Homo sapiens
3 The mechanism responsible for the phenobarbital increment in the rate of transcription of cytochrome P-450 b,e genes is unknown. Phenobarbital cytochrome P450 family 4 subfamily F member 3 Homo sapiens
4 The objective of this study was to assess whether active protein synthesis was needed for phenobarbital to induce the liver cytochrome P-450 b,e genes. Phenobarbital cytochrome P450 family 4 subfamily F member 3 Homo sapiens
5 While phenobarbital increased cytochrome P-450 b,e mRNAs about 12-fold at 3 hr, this induction was abolished by cycloheximide. Phenobarbital cytochrome P450 family 4 subfamily F member 3 Homo sapiens
6 To define whether the absence of protein synthesis in hepatocytes inhibited the phenobarbital induction of cytochrome P-450 at the transcriptional level, in vitro transcription rates using isolated nuclei were measured. Phenobarbital cytochrome P450 family 4 subfamily F member 3 Homo sapiens
7 After phenobarbital administration, there was about a 20-fold increment in transcriptional rate of cytochrome P-450 b,e genes. Phenobarbital cytochrome P450 family 4 subfamily F member 3 Homo sapiens
8 It is proposed that either preexisting regulatory proteins or transacting factors dependent on active protein synthesis participate in the regulation of liver cytochrome P-450 b,e gene transcription after phenobarbital. Phenobarbital cytochrome P450 family 4 subfamily F member 3 Homo sapiens