Pub. Date : 2021 Mar
PMID : 33190426
3 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
1 | In the present study, we evaluated an anti-HER2 scFv, which is specific for human epidermal growth receptor 2 (HER2) overexpressed in breast cancer, for functional expression in the cytoplasm of E. coli strains BL21(DE3) and SHuffle T7 Express, the latter of which is genetically engineered for cytoplasmic disulfide bond formation. | Disulfides | erb-b2 receptor tyrosine kinase 2 | Homo sapiens |
2 | In the present study, we evaluated an anti-HER2 scFv, which is specific for human epidermal growth receptor 2 (HER2) overexpressed in breast cancer, for functional expression in the cytoplasm of E. coli strains BL21(DE3) and SHuffle T7 Express, the latter of which is genetically engineered for cytoplasmic disulfide bond formation. | Disulfides | erb-b2 receptor tyrosine kinase 2 | Homo sapiens |
3 | We also found that SHuffle T7 Express cells were capable of supporting high-level soluble production of anti-HER2 scFvs with intact disulfide bonds independent of variable domain orientation, providing further evidence that SHuffle T7 Express is a promising host for laboratory and preparative expression of functional scFv antibodies. | Disulfides | erb-b2 receptor tyrosine kinase 2 | Homo sapiens |