Pub. Date : 2021 Feb
PMID : 33075181
15 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Blocking Von Willebrand Factor Free-Thiols inhibits binding to collagen under high and pathological shear stress. | Sulfhydryl Compounds | von Willebrand factor | Homo sapiens |
| 2 | BACKGROUND: Von Willebrand Factor (VWF) contains a number of free thiols, the majority of which are located in its C-domains, and these have been shown to alter VWF function, However, the impact of free-thiols on function following acute exposure of VWF to collagen under high and pathological shear stress has not been determined. | Sulfhydryl Compounds | von Willebrand factor | Homo sapiens |
| 3 | BACKGROUND: Von Willebrand Factor (VWF) contains a number of free thiols, the majority of which are located in its C-domains, and these have been shown to alter VWF function, However, the impact of free-thiols on function following acute exposure of VWF to collagen under high and pathological shear stress has not been determined. | Sulfhydryl Compounds | von Willebrand factor | Homo sapiens |
| 4 | BACKGROUND: Von Willebrand Factor (VWF) contains a number of free thiols, the majority of which are located in its C-domains, and these have been shown to alter VWF function, However, the impact of free-thiols on function following acute exposure of VWF to collagen under high and pathological shear stress has not been determined. | Sulfhydryl Compounds | von Willebrand factor | Homo sapiens |
| 5 | BACKGROUND: Von Willebrand Factor (VWF) contains a number of free thiols, the majority of which are located in its C-domains, and these have been shown to alter VWF function, However, the impact of free-thiols on function following acute exposure of VWF to collagen under high and pathological shear stress has not been determined. | Sulfhydryl Compounds | von Willebrand factor | Homo sapiens |
| 6 | BACKGROUND: Von Willebrand Factor (VWF) contains a number of free thiols, the majority of which are located in its C-domains, and these have been shown to alter VWF function, However, the impact of free-thiols on function following acute exposure of VWF to collagen under high and pathological shear stress has not been determined. | Sulfhydryl Compounds | von Willebrand factor | Homo sapiens |
| 7 | BACKGROUND: Von Willebrand Factor (VWF) contains a number of free thiols, the majority of which are located in its C-domains, and these have been shown to alter VWF function, However, the impact of free-thiols on function following acute exposure of VWF to collagen under high and pathological shear stress has not been determined. | Sulfhydryl Compounds | von Willebrand factor | Homo sapiens |
| 8 | RESULTS: Blockade of VWF free-thiols reduced VWF mediated platelet capture to collagen in a shear dependent manner, with platelet capture virtually abolished above 5000s-1 and in regions of stenosis in microfluidic channels. | Sulfhydryl Compounds | von Willebrand factor | Homo sapiens |
| 9 | RESULTS: Blockade of VWF free-thiols reduced VWF mediated platelet capture to collagen in a shear dependent manner, with platelet capture virtually abolished above 5000s-1 and in regions of stenosis in microfluidic channels. | Sulfhydryl Compounds | von Willebrand factor | Homo sapiens |
| 10 | AFM measurements showed that thiol-blockade reduced the life-time and strength of the VWF-collagen bond. | Sulfhydryl Compounds | von Willebrand factor | Homo sapiens |
| 11 | Pulling simulations of the VWF-C4 domain demonstrated that with one or two reduced disulphide bonds the C4 domain has increased flexibility and the propensity to undergo free-thiol exchange. | Sulfhydryl Compounds | von Willebrand factor | Homo sapiens |
| 12 | CONCLUSIONS: We conclude that free-thiols in the C-domains of VWF enhance the flexibility of the molecule and enable it to withstand high shear forces following collagen binding, demonstrating a previously unrecognised role for VWF free-thiols. | Sulfhydryl Compounds | von Willebrand factor | Homo sapiens |
| 13 | CONCLUSIONS: We conclude that free-thiols in the C-domains of VWF enhance the flexibility of the molecule and enable it to withstand high shear forces following collagen binding, demonstrating a previously unrecognised role for VWF free-thiols. | Sulfhydryl Compounds | von Willebrand factor | Homo sapiens |
| 14 | CONCLUSIONS: We conclude that free-thiols in the C-domains of VWF enhance the flexibility of the molecule and enable it to withstand high shear forces following collagen binding, demonstrating a previously unrecognised role for VWF free-thiols. | Sulfhydryl Compounds | von Willebrand factor | Homo sapiens |
| 15 | CONCLUSIONS: We conclude that free-thiols in the C-domains of VWF enhance the flexibility of the molecule and enable it to withstand high shear forces following collagen binding, demonstrating a previously unrecognised role for VWF free-thiols. | Sulfhydryl Compounds | von Willebrand factor | Homo sapiens |