Pub. Date : 2020
PMID : 32713538
3 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Our approach capitalizes on the ability of PLDs to catalyze transphosphatidylation reactions with exogenous alcohols to generate phosphatidyl alcohols, lipids whose location and abundance report on the extent of PLD-mediated PA synthesis. | Alcohols | glycosylphosphatidylinositol specific phospholipase D1 | Homo sapiens |
| 2 | Our key innovation is to employ functionalized, "clickable," alcohols as PLD substrates, which enables subsequent tagging of the resultant phosphatidyl alcohols with fluorophores or other functional probes for detection via highly selective click chemistry reactions. | Alcohols | glycosylphosphatidylinositol specific phospholipase D1 | Homo sapiens |
| 3 | In this chapter, we describe this method, termed IMPACT (Imaging PLD Activity with Clickable Alcohols via Transphosphatidylation), which can be coupled to downstream analysis by fluorescence microscopy, flow cytometry, HPLC, or mass spectrometry. | Alcohols | glycosylphosphatidylinositol specific phospholipase D1 | Homo sapiens |