Title : Assay of Phospholipase D Activity by an Amperometric Choline Oxidase Biosensor.

Pub. Date : 2020 Feb 27

PMID : 32121031






1 Functional Relationships(s)
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1 To perform the assay, an aliquot of a PLD standard solution is typically added to borate buffer containing phosphatidylcholine at a certain concentration and the oxidation current of hydrogen peroxide is then measured at the rotating modified electrode by applying a detection potential of + 0.7 V vs. SCE. Phosphatidylcholines glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens