Title : The effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the transcriptome of aryl hydrocarbon receptor (AhR) knock-down porcine granulosa cells.

Pub. Date : 2020

PMID : 32002328






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1 The effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the transcriptome of aryl hydrocarbon receptor (AhR) knock-down porcine granulosa cells. Polychlorinated Dibenzodioxins aryl hydrocarbon receptor Homo sapiens
2 The effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the transcriptome of aryl hydrocarbon receptor (AhR) knock-down porcine granulosa cells. Polychlorinated Dibenzodioxins aryl hydrocarbon receptor Homo sapiens
3 The effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the transcriptome of aryl hydrocarbon receptor (AhR) knock-down porcine granulosa cells. Polychlorinated Dibenzodioxins aryl hydrocarbon receptor Homo sapiens
4 The effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the transcriptome of aryl hydrocarbon receptor (AhR) knock-down porcine granulosa cells. Polychlorinated Dibenzodioxins aryl hydrocarbon receptor Homo sapiens
5 The well-characterized canonical mechanism of TCDD action involves the activation of aryl hydrocarbon receptor (AhR) pathway, but AhR-independent mechanisms were also suggested. Polychlorinated Dibenzodioxins aryl hydrocarbon receptor Homo sapiens
6 The well-characterized canonical mechanism of TCDD action involves the activation of aryl hydrocarbon receptor (AhR) pathway, but AhR-independent mechanisms were also suggested. Polychlorinated Dibenzodioxins aryl hydrocarbon receptor Homo sapiens
7 The well-characterized canonical mechanism of TCDD action involves the activation of aryl hydrocarbon receptor (AhR) pathway, but AhR-independent mechanisms were also suggested. Polychlorinated Dibenzodioxins aryl hydrocarbon receptor Homo sapiens
8 By applying RNA interference technology and Next Generation Sequencing (NGS) we aimed to identify genes involved in the mechanism of TCDD action in AhR knock-down porcine granulosa cells. Polychlorinated Dibenzodioxins aryl hydrocarbon receptor Homo sapiens
9 After transfection, medium was exchanged and the AhR knock-down cells were treated with TCDD (100 nM) for 3, 12 or 24 h, total cellular RNA was isolated and designated for NGS. Polychlorinated Dibenzodioxins aryl hydrocarbon receptor Homo sapiens
10 In TCDD-treated AhR knock-down cells we identified 360 differentially expressed genes (DEGs; P-adjusted < 0.05 and log2 fold change [log2FC] >= 1.0). Polychlorinated Dibenzodioxins aryl hydrocarbon receptor Homo sapiens
11 The three-time point study demonstrated that TCDD-induced changes in the transcriptome of AhR knock-down porcine granulosa cells were especially pronounced during the early stages of the treatment (3 h). Polychlorinated Dibenzodioxins aryl hydrocarbon receptor Homo sapiens
12 Conclusions: TCDD affected the transcriptome of AhR knock-down porcine granulosa cells. Polychlorinated Dibenzodioxins aryl hydrocarbon receptor Homo sapiens
13 The molecules involved in the AhR-independent action of TCDD were indicated in the study. Polychlorinated Dibenzodioxins aryl hydrocarbon receptor Homo sapiens