Title : CRISPR-Cas12a/Cpf1-assisted precise, efficient and multiplexed genome-editing in Yarrowia lipolytica.

Pub. Date : 2020 Jun

PMID : 31867213






2 Functional Relationships(s)
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1 To demonstrate the utility of CRISPR-Cpf1, we have optimized the CRISPR-Cpf1 system and achieved high-editing efficiency for two counter-selectable markers in the industrially-relevant oleaginous yeast Yarrowia lipolytica: arginine permease (93% for CAN1) and orotidine 5"-phosphate decarboxylase (~96% for URA3). Arginine Vasopressin Cbf1p Saccharomyces cerevisiae S288C
2 To demonstrate the utility of CRISPR-Cpf1, we have optimized the CRISPR-Cpf1 system and achieved high-editing efficiency for two counter-selectable markers in the industrially-relevant oleaginous yeast Yarrowia lipolytica: arginine permease (93% for CAN1) and orotidine 5"-phosphate decarboxylase (~96% for URA3). Arginine Vasopressin Cbf1p Saccharomyces cerevisiae S288C