Title : Expression, purification and stabilization of human serotonin transporter from E. coli.

Pub. Date : 2019 Dec

PMID : 31442583






1 Functional Relationships(s)
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1 CD spectroscopic analysis of protein stability allowed identifying CHS and POPX as stabilizing components, which increased hSERT thermostability by 7 C. The kinetic dissociation constant KD of 2.8 muM (+-0.05) for of the inhibitor Desipramine was determined with a ka of 10,848 M - 1 s-1 (+-220) and a kd of 0.03 s-1 (+-4.7 x 10-5). Desipramine solute carrier family 6 member 4 Homo sapiens