Title : Identification of alkaline pH optimum of human glucokinase because of ATP-mediated bias correction in outcomes of enzyme assays.

Pub. Date : 2019 Aug 6

PMID : 31388064






3 Functional Relationships(s)
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1 Identification of alkaline pH optimum of human glucokinase because of ATP-mediated bias correction in outcomes of enzyme assays. Adenosine Triphosphate glucokinase Homo sapiens
2 We demonstrated how the presence of ATP affects pH of widely used enzyme assay buffers and inversely affected KM of human hexokinase 2 and S0.5 of human glucokinase. Adenosine Triphosphate glucokinase Homo sapiens
3 We found that previously reported optimum of mammalian glucokinase was incorrect, affected by the ATP-induced pH shifts. Adenosine Triphosphate glucokinase Homo sapiens