Title : [Detection and characteristics of membrane form of phenylalanine hydroxylase (EC 1.14.16.1) from the rat liver].

Pub. Date : 1988 Jan

PMID : 3129035






1 Functional Relationships(s)
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1 The Km value of phenylalanine for the cytoplasmic form of phenylalanine hydroxylase is 0.32.10(-3) M, that for the membrane form is 1.66.10(-3) M. Both enzyme forms can bind to phenyl-Sepharose after their activation by the substrate, and they dissociate from the carrier after phenylalanine removal from the incubation mixture, which points to the intactness of the phenylalanine binding allosteric center in the membrane form of the enzyme. Phenylalanine phenylalanine hydroxylase Rattus norvegicus