Title : Production and characterization of polyclonal and monoclonal antibodies to rat brain L-glutamate decarboxylase.

Pub. Date : 1986 May 14

PMID : 3087572






1 Functional Relationships(s)
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1 The specificity of monoclonal antibodies against GAD were established from the following criteria: positive on ELISA test using homogeneous GAD as antigen; formation of GAD--anti-GAD IgG complex as indicated from gel filtration chromatography and sodium dodecyl sulfate polyacrylamide gel electrophoresis; and specific recognition of GAD subunit in a partially purified GAD preparation in Western immunoblotting test. Sodium Dodecyl Sulfate glutamate-ammonia ligase Rattus norvegicus