Pub. Date : 2018
PMID : 30588010
10 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Celastrol induces ubiquitin-dependent degradation of mTOR in breast cancer cells. | celastrol | mechanistic target of rapamycin kinase | Homo sapiens |
| 2 | Celastrol inhibits PI3K-Akt-mTOR signaling, which is frequently dysregulated in tumors and critical for tumor-cell proliferation and survival, but the underlying mechanisms are still not fully understood. | celastrol | mechanistic target of rapamycin kinase | Homo sapiens |
| 3 | In the present study, we investigated detailed mechanisms of celastrol inhibition of mTOR signaling in breast cancer cells. | celastrol | mechanistic target of rapamycin kinase | Homo sapiens |
| 4 | Second, we examined the effects of celastrol on mTOR phosphorylation and expression using Western blot. | celastrol | mechanistic target of rapamycin kinase | Homo sapiens |
| 5 | Furthermore, we investigated the cause of mTOR downregulation by celastrol using immunoprecipitation assays. | celastrol | mechanistic target of rapamycin kinase | Homo sapiens |
| 6 | Celastrol inhibited mTOR phosphorylation and induced mTOR ubiquitination, resulting in its proteasomal degradation. | celastrol | mechanistic target of rapamycin kinase | Homo sapiens |
| 7 | Celastrol inhibited mTOR phosphorylation and induced mTOR ubiquitination, resulting in its proteasomal degradation. | celastrol | mechanistic target of rapamycin kinase | Homo sapiens |
| 8 | Mechanistically, we found that mTOR is a client of Hsp90-Cdc37 chaperone complex, and celastrol disrupts mTOR interaction with chaperone Hsp90 while promoting mTOR association with cochaperone Cdc37. | celastrol | mechanistic target of rapamycin kinase | Homo sapiens |
| 9 | Mechanistically, we found that mTOR is a client of Hsp90-Cdc37 chaperone complex, and celastrol disrupts mTOR interaction with chaperone Hsp90 while promoting mTOR association with cochaperone Cdc37. | celastrol | mechanistic target of rapamycin kinase | Homo sapiens |
| 10 | Conclusion: Our study reveals that celastrol suppresses mTOR signaling, at least in part through regulating its association with chaperones and inducing its ubiquitination. | celastrol | mechanistic target of rapamycin kinase | Homo sapiens |