Title : Desensitizing plant EPSP synthase to glyphosate: Optimized global sequence context accommodates a glycine-to-alanine change in the active site.

Pub. Date : 2019 Jan 11

PMID : 30425098






4 Functional Relationships(s)
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Sentence
Compound Name
Protein Name
Organism
1 5-Enolpyruvylshikimate-3-phosphate synthase (EPSPS) catalyzes the transfer of a carboxyvinyl group from phosphoenolpyruvate (PEP) to shikimate-3-phosphate and in plants is the target of the herbicide glyphosate. glyphosate phosphoenolpyruvate carboxylase 2 Zea mays
2 A large portion of the reduction in Km was conferred by two down-sizing substitutions (L97C and V332A) within 8 A of glyphosate, which together reduced Km for PEP to 43 mum Although the original optimization was conducted with maize EPSPS, contextually homologous substitutions conferred similar properties to the EPSPSs of other crops. glyphosate phosphoenolpyruvate carboxylase 2 Zea mays
3 We also discovered a variant having the known glyphosate-desensitizing substitution P106L plus three additional ones that reduced the Km for PEP from 47 mum, observed with P106L alone, to 10.3 mum The improvements obtained with both Ala101 and Leu106 have implications regarding glyphosate-tolerant crops and weeds. glyphosate phosphoenolpyruvate carboxylase 2 Zea mays
4 We also discovered a variant having the known glyphosate-desensitizing substitution P106L plus three additional ones that reduced the Km for PEP from 47 mum, observed with P106L alone, to 10.3 mum The improvements obtained with both Ala101 and Leu106 have implications regarding glyphosate-tolerant crops and weeds. glyphosate phosphoenolpyruvate carboxylase 2 Zea mays