PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 30425098-1 2019 5-Enolpyruvylshikimate-3-phosphate synthase (EPSPS) catalyzes the transfer of a carboxyvinyl group from phosphoenolpyruvate (PEP) to shikimate-3-phosphate and in plants is the target of the herbicide glyphosate. glyphosate 200-210 phosphoenolpyruvate carboxylase 2 Zea mays 125-128 30425098-6 2019 A large portion of the reduction in Km was conferred by two down-sizing substitutions (L97C and V332A) within 8 A of glyphosate, which together reduced Km for PEP to 43 mum Although the original optimization was conducted with maize EPSPS, contextually homologous substitutions conferred similar properties to the EPSPSs of other crops. glyphosate 117-127 phosphoenolpyruvate carboxylase 2 Zea mays 159-162 30425098-7 2019 We also discovered a variant having the known glyphosate-desensitizing substitution P106L plus three additional ones that reduced the Km for PEP from 47 mum, observed with P106L alone, to 10.3 mum The improvements obtained with both Ala101 and Leu106 have implications regarding glyphosate-tolerant crops and weeds. glyphosate 46-56 phosphoenolpyruvate carboxylase 2 Zea mays 141-144 30425098-7 2019 We also discovered a variant having the known glyphosate-desensitizing substitution P106L plus three additional ones that reduced the Km for PEP from 47 mum, observed with P106L alone, to 10.3 mum The improvements obtained with both Ala101 and Leu106 have implications regarding glyphosate-tolerant crops and weeds. glyphosate 279-289 phosphoenolpyruvate carboxylase 2 Zea mays 141-144