Pub. Date : 2018 Feb 5
PMID : 29132113
3 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | The new approach takes advantage of Forster Resonance Energy Transfer (FRET) between, as the donor, a fluorescein-like diarsenical probe able to covalently bind a tetracysteine motif fused to the recombinant PDE5 catalytic domain and, as the acceptor, a rhodamine probe covalently bound to the pseudosubstrate cGMPS. | Cyclic GMP | phosphodiesterase 5A | Homo sapiens |
| 2 | The FRET efficiency decreases when a competitive ligand binds the PDE5 catalytic site and displaces the cGMPS-rhodamine conjugate. | Cyclic GMP | phosphodiesterase 5A | Homo sapiens |
| 3 | We have structurally investigated the PDE5/cGMPS-rhodamine complex by molecular modelling and have used the FRET signal to quantitatively characterize its binding equilibrium. | Cyclic GMP | phosphodiesterase 5A | Homo sapiens |