Title : Interaction between repressor Opi1p and ER membrane protein Scs2p facilitates transit of phosphatidic acid from the ER to mitochondria and is essential for INO1 gene expression in the presence of choline.

Pub. Date : 2017 Nov 10

PMID : 28924045






5 Functional Relationships(s)
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1 Inositol depletion favors Opi1p interaction with both Scs2p and phosphatidic acid at the endoplasmic reticulum (ER) membrane. Inositol phosphatidylinositol-binding protein SCS2 Saccharomyces cerevisiae S288C
2 Inositol supplementation, however, favors the translocation of Opi1p from the ER into the nucleus, where it interacts with the Ino2p-Ino4p complex, attenuating transcription of INO1 A strain devoid of Scs2p (scs2Delta) and a mutant, OPI1FFAT, lacking the ability to interact with Scs2p were utilized to examine the specific role(s) of the Opi1p-Scs2p interaction in the regulation of INO1 expression and overall lipid metabolism. Inositol phosphatidylinositol-binding protein SCS2 Saccharomyces cerevisiae S288C
3 Inositol supplementation, however, favors the translocation of Opi1p from the ER into the nucleus, where it interacts with the Ino2p-Ino4p complex, attenuating transcription of INO1 A strain devoid of Scs2p (scs2Delta) and a mutant, OPI1FFAT, lacking the ability to interact with Scs2p were utilized to examine the specific role(s) of the Opi1p-Scs2p interaction in the regulation of INO1 expression and overall lipid metabolism. Inositol phosphatidylinositol-binding protein SCS2 Saccharomyces cerevisiae S288C
4 Inositol supplementation, however, favors the translocation of Opi1p from the ER into the nucleus, where it interacts with the Ino2p-Ino4p complex, attenuating transcription of INO1 A strain devoid of Scs2p (scs2Delta) and a mutant, OPI1FFAT, lacking the ability to interact with Scs2p were utilized to examine the specific role(s) of the Opi1p-Scs2p interaction in the regulation of INO1 expression and overall lipid metabolism. Inositol phosphatidylinositol-binding protein SCS2 Saccharomyces cerevisiae S288C
5 Loss of the Opi1p-Scs2p interaction reduced INO1 expression and conferred inositol auxotrophy. Inositol phosphatidylinositol-binding protein SCS2 Saccharomyces cerevisiae S288C