Title : In situ activity and phosphorylation of tyrosine hydroxylase in the median eminence.

Pub. Date : 1986 Jun

PMID : 2872094






3 Functional Relationships(s)
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1 The in situ activity of TH was assayed by the accumulation of L-dihydroxyphenylalanine (DOPA) in the median eminence of hypothalamic fragments incubated in the presence of NSD 1015. Levodopa tyrosine hydroxylase Rattus norvegicus
2 The in situ activity of TH was assayed by the accumulation of L-dihydroxyphenylalanine (DOPA) in the median eminence of hypothalamic fragments incubated in the presence of NSD 1015. Levodopa tyrosine hydroxylase Rattus norvegicus
3 When hypothalamic fragments were incubated with veratridine (0-10(-3) M), maximal stimulation of TH activity was observed at 10(-4) M. The mean concentration of DOPA in the median eminence of hypothalamic fragments incubated with 10(-4) M veratridine was 3 times that seen in its absence. Levodopa tyrosine hydroxylase Rattus norvegicus