Title : Secretory expression, purification and functional characterization of 17β-hydroxysteroid dehydrogenase type 1 from mammalian HEK293T cells.

Pub. Date : 2017 Sep

PMID : 28669870






1 Functional Relationships(s)
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Protein Name
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1 The recombinant protein was purified by immobilized metal ion affinity chromatography yielding above 17 mg of purified 17beta-HSD1 protein per liter of cell culture, with a specific activity of 8.54 mumoL/min/mg of protein for conversion of estradiol into estrone, with NAD+ as cofactor at pH 9.2. NAD hydroxysteroid 17-beta dehydrogenase 1 Homo sapiens