PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 28669870-5 2017 The recombinant protein was purified by immobilized metal ion affinity chromatography yielding above 17 mg of purified 17beta-HSD1 protein per liter of cell culture, with a specific activity of 8.54 mumoL/min/mg of protein for conversion of estradiol into estrone, with NAD+ as cofactor at pH 9.2. NAD 270-274 hydroxysteroid 17-beta dehydrogenase 1 Homo sapiens 119-130