Title : Bioorthogonal Protein Conjugation: Application to the Development of a Highly Sensitive Bioluminescent Immunoassay for the Detection of Interferon-γ.

Pub. Date : 2017 Jun 21

PMID : 28514139

1 Functional Relationships(s)
Compound Name
Protein Name
1 A bioorthogonal conjugation approach is used to conjugate an anti-IFN-gamma antibody with a GLuc mutant containing the N-terminal tyrosine using formylbenzene diazonium hexafluorophosphate reagent (FBDP) in hydrophilic mild pH environment yielding high conjugation efficiency (60%). Tyrosine interferon gamma Homo sapiens