Title : 2-Methoxyestradiol blocks the RhoA/ROCK1 pathway in human aortic smooth muscle cells.

Pub. Date : 2015 Dec 15

PMID : 26487003






14 Functional Relationships(s)
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1 2-Methoxyestradiol blocks the RhoA/ROCK1 pathway in human aortic smooth muscle cells. 2-Methoxyestradiol ras homolog family member A Homo sapiens
2 Because the RhoA/ROCK1 pathway (RhoA ROCK1 myosin phosphatase targeting subunit myosin light chain) is involved in cytokinesis, herein we tested the concept that 2-ME also blocks the RhoA/ROCK1 pathway. 2-Methoxyestradiol ras homolog family member A Homo sapiens
3 Because the RhoA/ROCK1 pathway (RhoA ROCK1 myosin phosphatase targeting subunit myosin light chain) is involved in cytokinesis, herein we tested the concept that 2-ME also blocks the RhoA/ROCK1 pathway. 2-Methoxyestradiol ras homolog family member A Homo sapiens
4 Because the RhoA/ROCK1 pathway (RhoA ROCK1 myosin phosphatase targeting subunit myosin light chain) is involved in cytokinesis, herein we tested the concept that 2-ME also blocks the RhoA/ROCK1 pathway. 2-Methoxyestradiol ras homolog family member A Homo sapiens
5 Microarray transcriptional profiling suggested an effect of 2-ME on the RhoA/ROCK1 pathway. 2-Methoxyestradiol ras homolog family member A Homo sapiens
6 Indeed, 2-ME blocked mitogen-induced GTP-bound RhoABC expression and membrane-bound RhoA, suggesting interference with the activation of RhoA. 2-Methoxyestradiol ras homolog family member A Homo sapiens
7 Indeed, 2-ME blocked mitogen-induced GTP-bound RhoABC expression and membrane-bound RhoA, suggesting interference with the activation of RhoA. 2-Methoxyestradiol ras homolog family member A Homo sapiens
8 2-ME also reduced ROCK1 expression, suggesting reduced production of the primary downstream signaling kinase of the RhoA pathway. 2-Methoxyestradiol ras homolog family member A Homo sapiens
9 Moreover, 2-ME inhibited RhoA/ROCK1 pathway downstream signaling, including phosphorylated myosin phosphatase targeting subunit and myosin light chain; the ROCK1 inhibitor H-1152 mimicked these effects of 2-ME; both 2-ME and H-1152 blocked cytokinesis. 2-Methoxyestradiol ras homolog family member A Homo sapiens
10 Moreover, 2-ME inhibited RhoA/ROCK1 pathway downstream signaling, including phosphorylated myosin phosphatase targeting subunit and myosin light chain; the ROCK1 inhibitor H-1152 mimicked these effects of 2-ME; both 2-ME and H-1152 blocked cytokinesis. 2-Methoxyestradiol ras homolog family member A Homo sapiens
11 Moreover, 2-ME inhibited RhoA/ROCK1 pathway downstream signaling, including phosphorylated myosin phosphatase targeting subunit and myosin light chain; the ROCK1 inhibitor H-1152 mimicked these effects of 2-ME; both 2-ME and H-1152 blocked cytokinesis. 2-Methoxyestradiol ras homolog family member A Homo sapiens
12 2-ME also reduced the expression of tissue factor, yet another downstream signaling component of the RhoA/ROCK1 pathway. 2-Methoxyestradiol ras homolog family member A Homo sapiens
13 We conclude that 2-ME inhibits the pathway RhoA ROCK1 myosin phosphatase targeting subunit myosin light chain, and this likely contributes to the reduced cytokinesis in 2-ME treated HASMCs. 2-Methoxyestradiol ras homolog family member A Homo sapiens
14 We conclude that 2-ME inhibits the pathway RhoA ROCK1 myosin phosphatase targeting subunit myosin light chain, and this likely contributes to the reduced cytokinesis in 2-ME treated HASMCs. 2-Methoxyestradiol ras homolog family member A Homo sapiens