Title : Large-Scale Refolding and Enzyme Reaction of Human Preproinsulin for Production of Human Insulin.

Pub. Date : 2015 Oct

PMID : 26139616

1 Functional Relationships(s)
Compound Name
Protein Name
1 The most economical and optimal refolding condition for human preproinsulin was 1.5 g/l protein, 10 mM glycine buffer containing 0.6 M urea, pH 10.6, and 0.3 mM beta-mercaptoethanol at 15 C for 16 h. The maximum refolding yield was 74.8% at 15 C with 1.5 g/l protein. Glycine insulin Homo sapiens