Title : Real-time investigation of human topoisomerase I reaction kinetics using an optical sensor: a fast method for drug screening and determination of active enzyme concentrations.

Pub. Date : 2015 Jun 7

PMID : 25963854






4 Functional Relationships(s)
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1 The mechanism of hTopI is specifically targeted in cancer treatment using camptothecin derivatives. Camptothecin DNA topoisomerase I Homo sapiens
2 These drugs convert the hTopI activity into a cellular poison, and hence the cytotoxic effects of camptothecin derivatives correlate with the hTopI activity. Camptothecin DNA topoisomerase I Homo sapiens
3 These drugs convert the hTopI activity into a cellular poison, and hence the cytotoxic effects of camptothecin derivatives correlate with the hTopI activity. Camptothecin DNA topoisomerase I Homo sapiens
4 Here we demonstrate potential applications of a fluorophore-quencher based DNA sensor designed for measurement of hTopI cleavage-ligation activities, which are the catalytic steps affected by camptothecin. Camptothecin DNA topoisomerase I Homo sapiens