Title : Endotoxin depletion of recombinant protein preparations through their preferential binding to histidine tags.

Pub. Date : 2014 Dec 1

PMID : 25172132






2 Functional Relationships(s)
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1 We show that the thrombin-mediated cleavage of two histidine tags from the purified recombinant protein and the adsorption of these histidine tags and their associated endotoxins to a nickel affinity column result in an appreciable depletion of the endotoxins in the purified protein fraction. Histidine coagulation factor II, thrombin Homo sapiens
2 We show that the thrombin-mediated cleavage of two histidine tags from the purified recombinant protein and the adsorption of these histidine tags and their associated endotoxins to a nickel affinity column result in an appreciable depletion of the endotoxins in the purified protein fraction. Histidine coagulation factor II, thrombin Homo sapiens