Pub. Date : 1989 Jun 30
PMID : 2472218
8 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | EGF induces tyrosine phosphorylation of phospholipase C-II: a potential mechanism for EGF receptor signaling. | Tyrosine | phospholipase C gamma 1 | Homo sapiens |
| 2 | Binding of EGF to cells expressing human EGF receptor stimulated rapid tyrosine phosphorylation of phospholipase C-II (PLC-II), as revealed by immunoblotting analysis with phosphotyrosine-specific antibodies. | Tyrosine | phospholipase C gamma 1 | Homo sapiens |
| 3 | Binding of EGF to cells expressing human EGF receptor stimulated rapid tyrosine phosphorylation of phospholipase C-II (PLC-II), as revealed by immunoblotting analysis with phosphotyrosine-specific antibodies. | Tyrosine | phospholipase C gamma 1 | Homo sapiens |
| 4 | Tyrosine phosphorylation of PLC-II was stimulated by low physiological concentrations of EGF (1 nM), was quantitative, and was already maximal after a 30 sec incubation with 50 nM EGF at 37 degrees C. Interestingly, antibodies specific for PLC-II were able to coimmunoprecipitate the EGF receptor and antibodies against EGF receptor also coimmunoprecipitated PLC-II. | Tyrosine | phospholipase C gamma 1 | Homo sapiens |
| 5 | Tyrosine phosphorylation of PLC-II was stimulated by low physiological concentrations of EGF (1 nM), was quantitative, and was already maximal after a 30 sec incubation with 50 nM EGF at 37 degrees C. Interestingly, antibodies specific for PLC-II were able to coimmunoprecipitate the EGF receptor and antibodies against EGF receptor also coimmunoprecipitated PLC-II. | Tyrosine | phospholipase C gamma 1 | Homo sapiens |
| 6 | Tyrosine phosphorylation of PLC-II was stimulated by low physiological concentrations of EGF (1 nM), was quantitative, and was already maximal after a 30 sec incubation with 50 nM EGF at 37 degrees C. Interestingly, antibodies specific for PLC-II were able to coimmunoprecipitate the EGF receptor and antibodies against EGF receptor also coimmunoprecipitated PLC-II. | Tyrosine | phospholipase C gamma 1 | Homo sapiens |
| 7 | The protein tyrosine kinase inhibitor tyrphostin RG50864, which blocks EGF-dependent cell proliferation, blocked EGF-induced tyrosine phosphorylation of PLC-II, its association with EGF receptor, and EGF-induced Ca2+ release. | Tyrosine | phospholipase C gamma 1 | Homo sapiens |
| 8 | Hence, EGF-induced tyrosine phosphorylation of PLC-II may be a regulatory event linking the tyrosine kinase activity of EGF receptor to the PIP2 hydrolysis signaling pathway. | Tyrosine | phospholipase C gamma 1 | Homo sapiens |