Pub. Date : 2014
PMID : 24664372
12 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Celastrol blocks interleukin-6 gene expression via downregulation of NF-kappaB in prostate carcinoma cells. | celastrol | interleukin 6 | Homo sapiens |
| 2 | In this study, we evaluated the molecular mechanisms of celastrol on cell proliferation and IL-6 gene expression in prostate carcinoma cells. | celastrol | interleukin 6 | Homo sapiens |
| 3 | Knockdown of IL-6 attenuated the anti-proliferative effect of celastrol on PC-3 cells. | celastrol | interleukin 6 | Homo sapiens |
| 4 | Results from ELISA and 5"-deletion transient gene expression assays indicated that celastrol treatment decreased IL-6 secretion and gene expression, and this effect is dependent on the NF-kappaB response element within IL-6 promoter area since mutation of the NF-kappaB response element from AAATGTCCCATTTTCCC to AAATGTTACATTTTCCC by site-directed mutagenesis abolished the inhibition of celastrol on the IL-6 promoter activity. | celastrol | interleukin 6 | Homo sapiens |
| 5 | Results from ELISA and 5"-deletion transient gene expression assays indicated that celastrol treatment decreased IL-6 secretion and gene expression, and this effect is dependent on the NF-kappaB response element within IL-6 promoter area since mutation of the NF-kappaB response element from AAATGTCCCATTTTCCC to AAATGTTACATTTTCCC by site-directed mutagenesis abolished the inhibition of celastrol on the IL-6 promoter activity. | celastrol | interleukin 6 | Homo sapiens |
| 6 | Results from ELISA and 5"-deletion transient gene expression assays indicated that celastrol treatment decreased IL-6 secretion and gene expression, and this effect is dependent on the NF-kappaB response element within IL-6 promoter area since mutation of the NF-kappaB response element from AAATGTCCCATTTTCCC to AAATGTTACATTTTCCC by site-directed mutagenesis abolished the inhibition of celastrol on the IL-6 promoter activity. | celastrol | interleukin 6 | Homo sapiens |
| 7 | Results from ELISA and 5"-deletion transient gene expression assays indicated that celastrol treatment decreased IL-6 secretion and gene expression, and this effect is dependent on the NF-kappaB response element within IL-6 promoter area since mutation of the NF-kappaB response element from AAATGTCCCATTTTCCC to AAATGTTACATTTTCCC by site-directed mutagenesis abolished the inhibition of celastrol on the IL-6 promoter activity. | celastrol | interleukin 6 | Homo sapiens |
| 8 | Results from ELISA and 5"-deletion transient gene expression assays indicated that celastrol treatment decreased IL-6 secretion and gene expression, and this effect is dependent on the NF-kappaB response element within IL-6 promoter area since mutation of the NF-kappaB response element from AAATGTCCCATTTTCCC to AAATGTTACATTTTCCC by site-directed mutagenesis abolished the inhibition of celastrol on the IL-6 promoter activity. | celastrol | interleukin 6 | Homo sapiens |
| 9 | Celastrol also attenuated the activation of PMA and TNFalpha on the gene expression and secretion of IL-6 in PC-3 cells. | celastrol | interleukin 6 | Homo sapiens |
| 10 | Immunoblot assays revealed that celastrol treatment downregulated the expressions of IKKalpha, p50 and p65, supporting the 5"-deletion transient gene expression assay result that celastrol blocked IL-6 expression through the NF-kappaB pathway in PC-3 cells. | celastrol | interleukin 6 | Homo sapiens |
| 11 | Immunoblot assays revealed that celastrol treatment downregulated the expressions of IKKalpha, p50 and p65, supporting the 5"-deletion transient gene expression assay result that celastrol blocked IL-6 expression through the NF-kappaB pathway in PC-3 cells. | celastrol | interleukin 6 | Homo sapiens |
| 12 | For the first time, our results concluded that celastrol attenuates PC-3 cell proliferation via downregulation of IL-6 gene expression through the NF-kappaB-dependent pathway. | celastrol | interleukin 6 | Homo sapiens |