Pub. Date : 2014 Apr 2
PMID : 24548862
6 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Nicotine increases the resistance of lung cancer cells to cisplatin through enhancing Bcl-2 stability. | Nicotine | BCL2 apoptosis regulator | Homo sapiens |
| 2 | METHODS: In this study, we used immunoblotting and immunoprecipitation methods to test the ubiquitination and degradation of Bcl-2 affected by nicotine in lung cancer cells. | Nicotine | BCL2 apoptosis regulator | Homo sapiens |
| 3 | RESULTS: We demonstrated that the addition of nicotine greatly attenuated Bcl-2 ubiquitination and degradation, which further desensitised lung cancer cells to cisplatin-induced cytotoxicity. | Nicotine | BCL2 apoptosis regulator | Homo sapiens |
| 4 | In this process, Bcl-2 was persistently phosphorylated in the cells cotreated with nicotine and cisplatin. | Nicotine | BCL2 apoptosis regulator | Homo sapiens |
| 5 | Furthermore, Akt was proven to be responsible for sustained activation of Bcl-2 by nicotine, which further antagonised cisplatin-mediated apoptotic signalling. | Nicotine | BCL2 apoptosis regulator | Homo sapiens |
| 6 | CONCLUSIONS: Our study suggested that nicotine activates its downstream signalling to interfere with the ubiquitination process and prevent Bcl-2 from being degraded in lung cancer cells, resulting in the increase of chemoresistance. | Nicotine | BCL2 apoptosis regulator | Homo sapiens |